Fig. 2

ALKBH5-HOXA10 loop maintains ALKBH5 and HOXA10 overexpression in EOC. (a) The results of the RIP-qPCR assay show that HOXA10 mRNA is enriched in Flag-specific antibody in EOC cells on transfection with oe-ALKBH5 using Flag-tag. (b) The Act-D assay shows that ALKBH5 overexpression can maintain HOXA10 mRNA stability in EOC cells. (c and d) ALKBH5 regulates HOXA10 expression in EOC. (e) The ChIP-qPCR assay suggests that the ALKBH5 promoter fragment is enriched in HOXA10 (top), and the AGE assay validates the sonicated products and immunoprecipitated DNA of the ChIP assay (middle). EOC cells are transfected with HOXA10-Flag-tag (bottom). (f) Based on the TF binding motif of HOXA10 predicted by the JASPAR database (top), the luciferase reporter assay is conducted, and the results show that HOXA10 could interact with the region containing TAAA of ALKBH5 promoter (bottom). (g) The transfection efficiency of oe-HOXA10 lentivirus in A2780 and HO8910. (h) ALKBH5 is up-regulated in EOC cells with HOXA10 overexpression. (i) The transfection efficiency of specific siRNAs targeting HOXA10. (j) ALKBH5 expression is down-regulated by knocking down HOXA10 in EOC cells