Fig. 3

Gene expression signature of cryopreserved pNK cells before or after expansion using RNA sequencing. Genome-wide transcriptional profiles of cryopreserved pNK cells before (Day 0) or after expansion (Day 14). a. The heatmap depicts clustering of 5471 differentially expressed genes on Day 14 as compared to Day 0 pNK cells (q-value< 0.05 and fold change> 2 or < 0.5). Each column represents the expression profiles of individual donors in each experimental group. Upregulated gene set at Day 0 (Category I) and Day 14 (Category II). Warm color (red) denotes an increase in gene expression whereas cold color (blue) indicates a decrease as compared to the average level of gene expression in Day 0 pNK cells. b. Gene Ontology Biological Process (GOBP) analysis of upregulated genes on Day 14 (q-value< 0.05). c. Heat maps representing genes (labels to the right of plots) clustered in GOBP NK activation, NK inhibition, cytotoxicity, chemokine, inflammatory cytokine, immune suppression, anti-apoptosis, and proliferation (labels to the left of plots) with scaled intensities. Warm color (red) denotes an increase in gene expression whereas cold color (blue) indicates a decrease as compared to the average level of gene expression in Day 0 pNK cells. d. Volcano plot of the RNA-seq data (q-value< 0.05 and fold change> 2 or < 0.5)