Fig. 6

TAGLN regulates ovarian cancer progression through RhoA/ROCK pathway. A Western blot analyzed TAGLN, ROCK1, ROCK2 and RhoA of cells transfected with siControl or TAGLN siRNA. GAPDH was used as a loading control. B Western blot analyzed TAGLN, ROCK1, ROCK2 and RhoA of cells transfected with vector or TAGLN plasmid. GAPDH was used as a loading control. C-D Representative images (C) and scores (D)of IHC staining for ROCK1, ROCK2 and RhoA from mice bearing SK-OV-3 cells transfected with sh-control or sh-TAGLN lentivirus (*P < 0.05). Scale bar: 20 μm. E-F Migratory (E) and invasive (F) ability of cells treated with Rhosin or Y27632 after transfected with control vector or TAGLN plasmid (*P < 0.05, **P < 0.01, ****P < 0.0001). G A graphical illustration of the molecular signaling events involved in stiffness-regulated OC progression. TAGLN mediated stiffness-regulated OC progression through RhoA/ROCK pathway