Fig. 6

The relationship among circHMGCS1–016, CD73 and GAL-8 in ICC tissues. A. CD73 and GAL-8 levels in 40 pairs of ICC and matched adjacent non-tumor tissues. Data was shown as log₂^(T/N); B. Representative images for CD73 and GAL-8 staining of ICC and matched adjacent nontumor tissues (Bar = 100 μm); C. Representative images for circHMGCS1–016, CD73, GAL-8, CD4 and CD8 staining of ICC tissues (Bar = 200 μm); D. Correlation analysis showed the positive relationship between circHMGCS1–016 and CD73(R² = 0.7379, p = 3.45E-18), circHMGCS1–016 and GAL-8 (R² = 0.6747, p = 5.87E-16). But circHMGCS1_016 expression was adversely related to the level of CD8 (R² = 0.1299, p = 6.45E-17) and CD4 (R² = 0.2157, p = 1.17E-14). The relationship between CD8 and CD73(R² = 0.3711, p = 1.81E-12), CD8 and GAL-8 (R² = 0. 4244, p = 2.12E-23) was also analyzed (*** p < 0.001)