Fig. 6

FLVCR1-AS1 serves as a ceRNA to modulate KLF10 expression in PC. A The correlation between KLF10 and miR-513c-5p and miR-514b-5p expression in 77 PC tissue samples. B KLF10 expression in PANC-1 and PATU-8988 cells after transfection with miR-513c-5p or miR-514b-5p mimics. C Luciferase activity in 293T cells co-transfected with KLF10 wild-type or mutant sequence and miR-513c-5p or miR-514b-5p mimics. D The correlation between FLVCR1-AS1 and KLF10 expression levels in 77 PC tissue samples. E-F KLF10 expression was evaluated by qRT-PCR and western blotting in PANC-1 and PATU-8988 cells transfected with pcDNA-FLVCR1-AS1, miR-513c-5p, and miR-514b-5p mimics. G Luciferase activity of Luc-KLF10 with indicated treatment in 293T cells. H KLF10, PTEN, and p-AKT protein levels were detected by western blotting in PC cells with indicated treatment. I FLVCR1-AS1 mRNA expression was evaluated by qRT-PCR in PANC-1 cells with indicated treatment. *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance. All experiments were repeated three times