Fig. 4

Effects of E2 (10 nM) plus P4, mifepristone and metapristone on the expressions of cell adhesion molecules analyzed by flow cytometry, MMP-2, MMP-9, TIMP-1 and TIMP-2 analyzed by RT-PCR, and western blotting in JEG-3 and MCF-7 cells. E2 (10 nM) plus P4 increased the expression of integrinβ1, integrinα5, and EpCAM in JEG-3 cells (A), and increased the expression of integrinβ1, integrinα5, and sLex in MCF-7 cells (C). Mifepristone and metapristone decreased the expressions of sLex in both JEG-3 cells (B) and MCF-7 cells (D). Quantitative RT-PCR analysis of expressions of MMP-2, MMP-9, TIMP-1, TIMP-2 by JEG-3 (E) and MCF-7 cells (F) treated with E2 plus P4 at different concentrations. The changes in mRNA levels of each gene induced by E2 plus P4 were shown based on the control. Western blotting and related quantitative analysis showed changes in MMP-2 of JEG-3 cells (G), and MMP-2, MMP-9 in MCF-7 cells (H) induced by E2 plus P4 treatment. RT-PCR analysis showed changes in expressions of MMP-2 and TIMP-2 in JEG-3 cells (I), and of MMP-2 and MMP-9 in MCF-7 cells (J) induced by mifepristone and metapristone. Each bar represents the mean ± SEM (n= 3); *, P< 0.05; **, P< 0.01, compared with the control