Fig. 4
SMYD3 is one of the major regulators that drive INMT expression in CRPC. A Western blot for the levels of histone modification markers in purified primary PPC and CRPC cells of Myc-CaP allograft tumors. B ChIP assay for H3K4me3 binding to INMT promoter in purified primary PPC and CRPC cells of Myc-CaP allograft tumors. C ChIP assay for SMYD3 binding to INMT promoter in purified primary PPC and CRPC cells of Myc-CaP allograft tumors. D Western blot for INMT expression in Myc-CaP cells treated with vehicle or BCl-121 (60 μM) for 8 days in medium with normal or charcoal-treated FBS. E Western blot for INMT expression in LNCaP cells treated with vehicle or BCl-121 (60 μM) for 8 days in medium with normal or charcoal-treated FBS. F-H 1 × 106 Myc-CaP cells were inoculated s.c. into the flank of castrated FVB male mice, 6 days later followed by treatment with vehicle or 20 mg/kg BCl-121 once a day (F). Twenty three days after cell inoculation mice were sacrificed and tumor were collected and weighted (G), and the expression of INMT in tumor tissues was detected by western blot (H). *, P < 0.05; **, P < 0.01