Fig. 5
Knockdown of INMT amplifies the anticancer activity of selenium compounds MSA and MSC in vitro and in mouse models. A MTT analysis for the viability of INMT-KD or control Myc-CaP cells cultured in charcoal-treated medium and treated with vehicle or MSA (1 μM) for 72 h. B MTT analysis for the viability of INMT-KD or control DU145 cells cultured in normal medium and treated with vehicle or MSA (1 μM) for 72 h. C, D 1 × 106 INMT-KD or control Myc-CaP cells were inoculated s.c. into the flank of castrated FVB male mice, 5 days later followed by treatment with i.p. vehicle or 5 mg/kg MSC once a day (C). Twenty six days after cell inoculation mice were sacrificed and tumor were collected and weighted (D). E 5 × 106 INMT-KD or control DU145 cells were inoculated s.c. into the flank of RAG1−/− male mice, 5 days later mice were treated with i.p. vehicle or 5 mg/kg MSC once a day. Tumor volume was determined at indicated intervals. *, P < 0.05; **, P < 0.01; ***, P < 0.001