Fig. 6
Knockdown of INMT enhances the anticancer activity of Bis(7)-Tacrine. A The structure of Bis(7)-Tacrine. B MTT analysis for the viability of INMT-KD and control Myc-CaP cells cultured in charcoal-treated medium and treated with vehicle or Bis(7)-Tacrine at different concentrations for 72 h. C MTT analysis for the cell viability of INMT-KD and control DU145 cells treated with vehicle or Bis(7)-Tacrine at different concentrations for 72 h. D, E 1 × 106 INMT-KD or control Myc-CaP cells were inoculated s.c. into the flank of castrated FVB male mice. Five days later mice were treated with i.v. vehicle or 2 mg/kg Bis(7)-Tacrine once every 2 days (D). 24 days after cell inoculation mice were sacrificed and tumors were collected and weighted (E). *, P < 0.05; **, P < 0.01; ***, P < 0.001