Fig. 3

TRAF1 acted as a direct target gene of miR-378a-3p in HCC cells. A RNA-seq identified differentially expressed genes between SMMC-7721-overexpressing and NC cells. B Venn diagram of the potential target of miR-378a-3p using two independent algorithms (miRWalk and miRTarBase) combined with our RNA-seq. C Prediction of the potential binding site of miR-378a-3p and TRAF1 through bioinformatics websites. D Relative luciferase activity in HCCLM3 and SMMC-7721 cells co-transfected with miR-378a-3p mimic and WT or MUT TRAF1. E, F TRAF1 mRNA and protein levels in HCCLM3 and SMMC-7721 cells. *P < 0.05; **P < 0.01; ***P < 0.001