Fig. 4

STOML2 interacts with PHB to modulate RAF/MEK/ERK MAPK pathway. a Yeast two-hybrid assay. Prey plasmids in 27 positive clones were extracted and sequenced. Sequences were looked up in PUBMED-BLAST for corresponding gene. “+” sign indicates counts of each gene from 29 positive clones. b Co-immunoprecipitation to validated STOML2 and DTYMK interaction in 293 T cells. c In vitro binding of STOML2 and PHB in co-IP analysis. d Immunostaining analysis of STOML2 (green), PHB (red) and DAPI (blue) in SW620-STOML2. Scale bar, 5 μm. e Formalin fixed paraffin embedded (FFPE) sections from CRC tumor-adjacent tissue were subjected to immunostaining analysis of STOML2 (green), PHB (red) and DAPI for DNA (blue). Scale bar, 20 μm (upper and lower row), 50 μm (middle row). f Association of STOML2, PHB, RAF1 and MAPK signaling pathway from STRING database. Interaction score: STOML2 and PHB (0.916); PHB and RAF1 (0.965); RAF1 and ERK (0.964); RAF1 and MEK (0.516); MEK and ERK (0.645). g Immunoblot analysis of STOML2, PHB and MAPK signaling pathway in SW620-scr and -shSTOML2 cells. h Immunoblot analysis of STOML2, PHB and MAPK signaling pathway in SW480-mock and –STOML2 cells