Fig. 4

Ov90 transcriptome profiling upon L1CAM ectopic expression and validation at protein level. (A) Hierarchical clusters of L1CAM regulated genes in Ov90-Bulk (n = 709) or Ov90-OCSC experimental conditions (n = 1289). (B) IPA-upstream modulator analyses of Ov90-bulk and Ov90-OCSC overexpressing L1CAM. The heatmap represents activation z-scores (≥1; p-value< 0.05) of the mechanisms shown on the left which were ordered from the higher (on top) to the lower (bottom) z-score value (average of the two experimental conditions). (C) Immunoblotting on L1CAM-transduced and control Ov90 cells, either as bulk or OCSC. α-tubulin served as loading control. (D) Immunoblotting on nuclear and cytosolic fractions of serum-starved Ov90-mock and Ov90-L1CAM. HDAC1 and α-tubulin served as nuclear and cytosolic loading control, respectively. (E) Sections of two representative OC samples co-stained for L1CAM (red) and pSTAT3 (brown). Arrows indicate L1CAM and pSTAT3 co-localization in tumor cells. Scale bar, 20 μm