Fig. 4
From: Arachidonic acid drives adaptive responses to chemotherapy-induced stress in malignant mesothelioma
AA treatment increased the number of ALDHbright MPM cells. A. Positive correlation between the CC50 of pemetrexed (y-axis) and the magnitude of AA release (expressed as folds over ctrl) in nine representative MPM cell lines (as from Fig. 2B, except that NCI-H2595 was not included because no significant increase in AA was recorded following pem treatment). Linear regression analysis with indicated r square (r2: 0.6243). B. Representative flow cytometry plots of NCI-H2373 (upper panels) and SPC-212 (lower panels) MPM cells treated for 48 h with AA 3 microMol/L or ctrl (saline), The percentage of ALDHbright cells was determined over the same cells treated with a specific ALDH inhibitor (DEAB) immediately after adding the ALDH substrate (BAAA). C. Histogram showing the percentage of ALDHbright cells treated as indicated in A, from additional representative MPM cell lines. Duplicate experiments. D. Histogram showing the relative levels of the ALDH1A3 mRNA detected by RT-QPCR in HP1, NCI-H2373, MSTO-211H cells treated with AA (3micromol/L), for 24 h. Folds over control-treated cells were reported. Statistics: * p < 0.05 except where indicated ns = not significant (p > 0.05). E. Heatmap showing the levels of the indicated mRNAs detected by RT-QPCR in HP1, NCI-H2373, MSTO-211H cells treated with AA (3microMol/L), for 24 and 48 h. Only mRNA significantly modulated (p < 0.05) at either 24 h or 48 h or both were included in the map