Fig. 5

SETD1A activates NEAT1 and EZH2 transcription to activate the Wnt/β-catenin pathway. A-B, The correlation between SETD1A and NEAT1, EZH2 was analyzed using GEPIA online tool. R, Pearson’s correlation coefficient. C, Schematic showing the ChIP-PCR detection site in the NEAT1 and EZH2 promoters. D, The enrichment of SETD1A and H3K4me3 in the NEAT1 and EZH2 promoters in A549 cells was detected by ChIP-PCR assay. E, The relative enrichment of SETD1A and H3K4me3 in the NEAT1 and EZH2 promoters was detected by ChIP-qPCR assay following SETD1A knockdown in A549 cells. F, The promoter activity was analyzed by luciferase activity assay in A549 cells. G-H, NEAT1 and EZH2 transcript levels in NSCLC cells were detected by qRT-PCR following SETD1A knockdown. I, EZH2 protein levels in NSCLC cells as indicated were detected by western blotting following SETD1A knockdown. J-K, NEAT1 and EZH2 transcript levels in NSCLC cells were detected by qRT-PCR following SETD1A overexpression. L, EZH2 protein levels in NSCLC cells were detected by western blotting following SETD1A overexpression. Data are shown as means ± SD. *P < 0.05, **P < 0.01.