Fig. 4

MUS81 inhibition amplifies cytosolic DNA accumulation and promotes innate immune activation in gastric cancer cells. a Gene Ontology analysis of MUS81-related differentially expressed genes (DEGs) in the GSE62254 data set. b Gene Set Enrichment Analysis of MUS81-related DEGs during immune response. P = 0.002, FDR q-value = 0.048. c and d Representative images of PicoGreen staining in shScramble or MUS81 knockdown (shMUS81#1 and shMUS81#2) SGC7901 and BGC823 cells treated with dimethyl sulfoxide (DMSO) or MK1775 (0.50 μM for SGC7901 and 0.25 μM for BGC823). Data are reported as mean ± SD for five independent experiments. e and f Western blot of phosphorylated TBK1 (p-TBK1) and total TBK1 treated with MK1775 (0.50 μM) in SGC7901 and MK1775 (0.25 μM) in BGC823 cells for 24 h. g–i qPCR of proinflammatory cytokines CCL20 and CXCL10 and IFN-β in SGC7901 cells treated with MK1775 (0.50 μM) for 12 h. The data are presented as mean ± SD (n = 3). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns., not significant