Fig. 8

HOTAIRM1 knockdown impairs leukemogenesis in vivo. a Blood was collected from all animals via the tail vein 4 weeks after cell transplantation, and the total WBC count was determined. b-c The mean liver (b) and spleen (c) weights of mice transplanted with shNC, shHOTAIRM1 and shHOTAIRM1 + HOTAIRM1 cells were quantified. d Immature cells from bone marrow were evaluated by Wright’s staining. e Representative images of histologic sections of livers and spleens from mice transplanted with cells treated as indicated. Scale bar: 50 μm. f IHC staining of Ki-67 in liver and spleen tissue from mice transplanted with cells. Scale bar: 50 μm. g The protein levels of LC3 and p62 in each group were determined by western blotting. h Kaplan-Meier survival curves of mice in each group