Fig. 5

MiR-1265 was downregulated in CRC and suppressed C2CD4A expression by directly binding to the 3’UTR of C2CD4A mRNA. a Venn diagram illustrated the overlap of miRNAs detected in the miRWalk, TargetScan and microRNA.org. b The relative expression levels of miR-1265 were independent experiments were performed for each by qRT-PCR in 63 pairs of fresh frozen CRC tissues and adjacent noncancer tissues. c The FISH results showed that the level of miR-1265 was lower in CRC tissues than that in adjacent normal mucosae tissues from the TMAs (n = 106). d, e 293 T, RKO, and HCT8 cells were transfected with mock, miRNA control and miR-1265 mimics or miR-1265 inhibitor; and the expression of C2CD4A were detected by qRT-PCR and western blot. f Schematic of C2CD4A 3’UTR wild-type (WT) and mutant (Mut) luciferase reporter vector are shown. g Wild type (WT) or mutant C2CD4A 3’-UTR were transfected into 293 T, RKO, HCT8 with miR-1265/NC or miR-1265 mimics or miR-1265/inhibitor. Luciferase reporter assay were performed to detect different groups luciferase activity, respectively. Mean ± SEM was shown for these data. Three independent experiments were performed for each group. (ns showed no significance. *P < 0.05, **P < 0.01, ***P < 0.001)