Fig. 3

EIF4A3 protein mediated circ_0004296-induced inhibition of host gene ETS1 expression in PCa cells. A, B Intracellular distribution of circ_0004296 was detected by RNA-FISH and nuclear-plasma extraction assay. Scale bars =50um. C The circ_0004296 overexpression decreased the expression of the ETS1 protein. D circ_0004296 knockdown increased the expression of the ETS1 protein. E Schematic illustration of RNA pull-down for circ_0004296. F Schematic exhibiting the overlap for specific proteins bound by circ_0004296 via protein profiling and the potential target genes of circ_0004296 predicted by CircInteractome and CircFunBase. G RNA pull-down and western blotting assays confirmed the interaction of circ_0004296 with EIF4A3. H The efficiency of EIF4A3 knockdown at mRNA level. I The efficiency of EIF4A3 knockdown at protein level. J EIF4A3 knockdown decreased ETS1 expression. *P < 0.05, **P < 0.01, ***P < 0.001