Fig. 2

Knockout of lnc-RP11-536 K7.3 increased chemosensitivity in chemo-resistant organoids and cells in CC patients. A Chemo-resistant RKO (CR-RKO) cell line was generated by gradual increase of oxaliplatin concentration. CCK8 assay showed that IC50 value of CR-RKO cells was much higher than that of the parental RKO cells (^**P < 0.01). B qRT-PCR assay was used to detect the efficacy of lnc-RP11-536 K7.3 knockout in CR-RKO cell lines. C Cell viability assay of organoids treated with or without 1 uM oxaliplatin in different time intervals. D IC50 values of oxaliplatin. Lnc-RP11-536 K7.3-knockout and control cells were treated with different concentrations of oxaliplatin for 48 h (^**P < 0.01). E-H Colony formation efficiency of chemo-resistant CC organoids and cells treated with or without 2 uM oxaliplatin for 7 days. Represent pictures in chemo-resistant CC organoiI (E) and chemo-resistant RKO cells (F). Statistical analysis of relative colony formation in chemo-resistant CC organoids (G) and chemo-resistant RKO cells (H) (^**P < 0.01). Above experiments were repeated 3 times