Fig. 7

CPZ-induced mitotic catastrophe in GBM cells is dependent on ER stress. Cell nuclei were stained with Hoechst and analyzed in control (CTL) and CPZ-treated cells, either in the presence or absence of the ER stress inhibitor 4-PBA in T98G, U-87 MG and U-251 MG anchorage-dependent GBM cells (A), TS#1, TS#83 and TS#163 neurospheres (B) and RPE-1 non-cancer cells (C). In each panel, left pictures are representative images, while histograms on the right highlight the relative number of normal (white) and aberrant (grey) nuclei counted in all the experimental sets