Fig. 6

Effect of Pladienolide-B on PDAC CSC functional properties. A Colony formation efficiency represented as crystal violet absorbance (AU; arbitrary units) in PDAC PDX-derived cells after Pladienolide-B treatment compared with vehicle-treated cells. Representative images of colony formation (n = 6). B Sphere formation efficiency (number of spheres/mL) in PDAC PDX-derived cells after Pladienolide-B treatment compared to vehicle-treated cells (n = 3). C, D Quantification of annexin-V staining in Pladienolide-B-treated cells across PDX-derived in vitro cultures compared with vehicle-treated cells. Top and bottom: live cells (blue); dead cells (black); early apoptosis (yellow) and late apoptosis (grey) (n = 1). E Cell death, measured with the bioluminescence Toxilight assay, after treatment with the indicated compounds in combination with Pladienolide-B (n = 4). Data represents mean ± SEM. Asterisks indicate significant differences (*p < 0.05; **p < 0.01; ***p < 0.001)