Fig. 4

LINC01050 knockdown inhibits gastric cancer cell proliferation and induces apoptosis in vitro and inhibits tumor growth in vivo. a qRT-PCR analysis of LINC01050 expression in BGC823 and KATO III cells transfected with si-NC (negative control), si-LINC01050#1, or si-LINC01050#2. Data are presented as mean ± SD (n = 3). ^**P < 0.01. b-c Proliferation of BGC823 and KATO III cells transfected with si-NC (negative control), si-LINC01050#1, or si-LINC01050#2, as determined using CCK8 (b) and EdU assays (c). Data are presented as mean ± SD (n = 3). ^**P < 0.01. d Colony-formation capabilities of BGC823 and KATO III cells transfected with si-NC (negative control), si-LINC01050#1, or si-LINC01050#2, as determined using plate colony formation assays. Data are presented as mean ± SD (n = 3). ^**P < 0.01. e Cell apoptosis in BGC823 and KATO III cells transfected with si-NC (negative control), si-LINC01050#1, or si-LINC01050#2 for 48 h, analyzed using flow cytometry by Annexin V-FITC and Propidium iodide (PI) staining. Data are presented as mean ± SD (n = 3). ^*P < 0.05. f Western blot analysis of cleaved PARP 1, cleaved Caspase-3, Bcl-2, and Bax expression. GAPDH was used as an internal control. Data are presented as mean ± SD (n = 3). ^*P < 0.05, ^**P < 0.01. g Growth curves of tumors from KATO III cells transduced with lentiviral sh-LINC01050 in tumor-bearing nude mice. Data are presented as mean ± SD (n = 5). ^*P < 0.05. h Weights of tumors from nude mice. The values are presented as the means ± SD (lower panel, n = 5). Three representative images of the tumors from the nude mice are shown (upper panel). ^*P < 0.05. i qRT-PCR analysis of miR-7161-3p expressions in subcutaneous tumor tissues of KATO III cells transduced with lentiviral shNC (negative control) or shLINC01050. Data are presented as mean ± SD (n = 3). *P < 0.05. j Western blot analysis of SPZ1 expressions in subcutaneous tumor tissues of KATO III cells transduced with lentiviral shNC or shLINC01050. GAPDH was used as an internal control