Fig. 4

ASB2 downregulation in DLBCL cell lines harvested 72 h after nucleofection. A MTT assay for DLBCL cell lines nucleofected with either 500 nM control (CNT) siRNA, FLI1 siRNA or ASB2 siRNA. B Normalized (to GAPDH) relative mRNA expression of ASB2 from CNT siRNA and ASB2 siRNA treated cells. C Immunoblot and its quantification showing protein expression of ASB2 and IκBα in DLBCL CNT siRNA, FLI1 siRNA and ASB2 siRNA treated cells. Mouse monoclonal α-GAPDH was used as loading control. For each figure two replicates were performed for each cell line