Fig. 5

FOSL2 binds to the promoter of SNHG17 and promotes its expression. a The JASPAR database was used to search the FOSL2 binding motif within the SNHG17 promoter. b RT-qPCR was used to verify the expression of SNHG17 and FOSL2 when FOSL2 was silenced. c The knockdown efficiency of FOSL2 was detected by western blotting. d The luciferase activity of the SNHG17 promoter (WT, #1-MUT, #2-MUT and #3-MUT) was tested via luciferase reporter assays when FOSL2 was overexpressed. Schematic depictions show the different luciferase reporter plasmids; blue represents the predicted binding sites between FOSL2 and the SNHG17 promoter; and red represents the mutant sites. e The luciferase activity of the SNHG17 promoter (WT and #2-MUT) was tested via luciferase reporter assays when FOSL2 was silenced. f ChIP assays were conducted to prove that FOSL2 could bind to the SNHG17 promoter in HCT116 cells. g The relative mRNA expression of FOSL2 was verified in cohort 1 CRC tissues and NCTs by RT-qPCR. h Bivariate correlation analysis between SNHG17 RNA expression and FOSL2 mRNA expression in cohort 1 CRC tissues.