Fig. 3

PGRMC1 expression induces ferroptosis. A and B Immunoblotting in HN4PCC with vector or shPGRMC1 transfection (A) and HN4 parental cells with control or PGRMC1 overexpression vector transfection (B). C-E Cell viability, lipid peroxidation, and labile iron pool in the PCC with vector or shPGRMC1. The cells were examined after treatment with ferroptosis inducers: 1 μM RSL3, 10 μM erastin, 0.5 mM SAS, and cyst(e)ine deprivation for 48 h for cell viability and 8 h for lipid peroxidation and labile iron pool. Data are means and s.d. from three technical replicates. *P < 0.05, **P < 0.01, ***P < 0.001 relative to vector control (vtr). F-H Cell viability, lipid peroxidation, and labile iron pool in HN4 parental cells and PGRMC1 overexpression cells were detected after treatment with ferroptosis inducers: 1 μM RSL3, 10 μM erastin, 0.5 mM SAS. *P < 0.05, **P < 0.01, ***P < 0.001 relative to vector control. I Immunoblotting in HN4 parental cells with control or PGRMC1 overexpression vector transfection and HN4PCC with vector or shPGRMC1 transfection