Fig. 6

BUB1 regulated the proliferation and invasion of T24 and 5637 bladder cancer cells. A. T24 cells were transfected with sicontrol or siBUB1, and an MTT assay was used to evaluate cell proliferation. B. 5637 cell were transfected with sicontrol or siBUB1, and an MTT assay was used to evaluate cell proliferation. C. 5637 and T24 cells were transfected with control, BUB1 wild-type or BUB1 K821R vector, and Transwell assay was used to evaluate cell invasion ability. D. 5637 and T24 cells were transfected with si control or siBUB1, and Transwell assays were used to detect cell invasion ability. E. 5637 cell were cotransfected with BUB1 siRNA and the CFP-H3 plasmid for 48 h. Live-cell imaging was performed to monitor chromosome segregation during mitosis. The differential interference contrast (DIC) and CFP channels were recorded every 10 min for 16 h. Representative still images are shown. Scale bar 5 μm