Fig. 8

BUB1 Inhibitor 2OH-BNPP1 suppressed BCa Tumor Growth. A T24 cell were treated with 2OH-BNPP1 (5 or 10 μM) or vehicle (10% DMSO) for 96 h, and the number of viable cells was determined by an MTT assay. B 5637 cell were treated with 2OH-BNPP1 or vehicle (10% DMSO) for 96 h, and the number of viable cells was determined by an MTT assay. C 5637 cell were treated with 2OH-BNPP1 or vehicle (10% DMSO) for 24 h, and the number of invading cells was determined by an invasion assay. D T24 cell were treated with 2OH-BNPP1 or vehicle (10% DMSO) for 24 h, and the number of invading cells was determined by an invasion assay. E 5637 cell were implanted subcutaneously into male BALB/c mice. When the tumors became palpable, the mice were injected with either vehicle (10% DMSO in PBS) or 2OH-BNPP1 (100 mg/kg of body weight) for 10 days. Tumors were measured with calipers. F Quantitation of Ki-67, p-stat3, NFATC2 and LHX1 expression in 5637 xenograft tumors from each group. Specimens were obtained 10 days post treatment. IHC staining was scored according to the number of cells expressing the indicated proteins, and statistical analysis was performed (nonparametric Kruskal–Wallis test) to determine significance. See also Supplementary Fig. 3. **p < 0.01, *p < 0.05