Fig. 6

CENP-N was regulated directly by IRF2 in NPC cells. a Sequence logo of the IRF2 binding site in the CENP-N promoter. b The GEPIA website was used to predict the correlation between IRF2 and CENP-N expression. c PCR analysis of the ChIP product showed that IRF2 bound to the CENP-N gene promoter region. d A luciferase reporter assay was used to detect the effects of IRF2 binding site in the promoter region of CENP-N. e Western blotting was used to verify the correlation between IRF2 and CENP-N expression. f Representative images from NPG and NPC tissue samples with low or high IRF2 immunohistochemical staining scores (4× and 200×). g Statistical analysis of the immunohistochemical results showed that IRF2 was highly expressed in NPC. The data are shown as the mean ± SD values. * p < 0.05, ** p < 0.01, ***p < 0.001