Fig. 5

ZIP10 decreases thyroid cancer cell responsiveness to mannose. a The proliferation of ZIP10 knockdown-8305C/8505C cells and control cells treated with 20 mM mannose treatment or not. b The proliferation of ZIP10 overexpression-TPC-1/FTC133 cells and control cells treated with 20 mM mannose or not. c Colony formation of ZIP10 knockdown-8305C/8505C cells and control cells treated with mannose in a dose-dependent manner. d Colony formation of ZIP10 overexpression-TPC-1/FTC133 cells and control cells treated with mannose in a dose-dependent manner. The representative colony images were shown in the upper panels. Colony numbers were then counted and presented in the lower panels. e ZIP10 knockdown-8305C and 8505C cells were treated with mannose or not for 24 h, and stained with PI. Cell cycle distributions were then analyzed by flow cytometry. f Western blot analysis of cell cycle-related molecules (including p53, cyclin E and cyclin D, pCDK2 and CDK2) in ZIP10 knockdown-8305C and 8505C after a 24-h mannose treatment. β-actin was used as a loading control. The data were presented as mean ± SD. Statistically significant differences were indicated: *, P < 0.05; **, P < 0.01; ***, P < 0.001