Fig. 7
Silencing TINCR impairs HCC tumorigenesis and resistance to oxaliplatin in vivo. A, TINCR expression in HepG2 and HuH7 cells transfected with stable sh-TINCRs or scrambled control. B-D, HuH7 cells with or without stable TINCR knockdown were transplanted into the axilla of nude mice. Once the tumor nodes grew to 100 mm3, the mice were randomly divided into four groups (n = 6/group) and intraperitoneally injected with 5% glucose solution (GS) or oxaliplatin (OXA) (3 mg/kg) every 3 days. Representative images (B), tumor volume growth curves (C), and weight of the xenograft tumors(D). E-F, Representative images (E) and quantification (F) of immunohistochemistry analysis of ST6GAL1 expression level in tumors from HuH7 cells stably transfected with sh-TINCR 2# and scramble control. Data are expressed as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; NS: no significance