Fig. 6
The effect of double targeting of NGF and autophagy on PNI. A. Dorsal root ganglion monoculture with the control, RO (1 μM), CQ (40 μM) or RO + CQ (1 μM + 40 μM). The intervention begins at 48 h, and light microscopy images were collected at 24 h, 48 h, and 72 h. Dashes indicate the neurofilament scope. B. Statistics of the migration distance (μm) of DRG neurofilaments treated with the control, RO, CQ or RO + CQ (cell image: Fig. 6A) (* p < 0.05). C. Statistics of the migration span (μm2) of DRG neurofilaments treated with the control, RO, CQ or RO + CQ (cell image: Fig. 6A) (* p < 0.05). D. DRGs cocultured with PANC-1 or BxPCc-3 cells were treated with control, RO (1 μM), CQ (40 μM) or RO + CQ (1 μM + 40 μM). Black dashes indicates the cancer line margin. Red dashes indicates the DRG neurofilament margin. E. Statistics of the migration distance (μm) of DRG-derived Schwann cells toward PANC-1 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 6D) (* p < 0.05). F. Statistics of the growth cone numbers of PANC-1 cells toward DRGs treated with the control, RO, CQ or RO + CQ (cell image: Fig. 6D) (* p < 0.05). G. Statistics of the migration distance (μm) of PANC-1 cells toward DRGs treated with the control, RO, CQ or RO + CQ (cell image: Fig. 6D) (* p < 0.05). H. Statistics of the migration distance (μm) of DRG-derived Schwann cells toward BxPC-3 cells treated with control, RO (1 μM), CQ (40 μM) or RO + CQ (1 μM + 40 μM) (* p < 0.05). I. Statistics of growth cone numbers of BxPCc-3 cells toward DRGs treated with the control, RO, CQ or RO + CQ (cell image: Fig. 6D) (* p < 0.05). J. Statistics of the migration distance (μm) of BxPC-3 cells toward DRGs treated with the control, RO, CQ or RO + CQ (cell image: Fig. 6D) (* p < 0.05).K. Statistics of the migration distance (μm) of PANC-1 cells toward DRGs treated with the control, RO, CQ or RO + CQ (cell image: Fig. 6D) (* p < 0.05)