Fig. 7

Double targeting of NGF and autophagy inhibits PNI in vivo. A. Body weight (g) of the nude mice with sciatic nerve PANC-1 injection treated with the control, RO, CQ or RO + CQ. No significant body weight difference was detected at day 1,7,14,21 and 28. B. Sciatic nerve score of the nude mice with sciatic nerve PANC-1 injection treated with the control, RO, CQ or RO + CQ. RO + CQ could increase the sciatic nerve score at day 28. C. The paw span (mm) of the nude mice with sciatic nerve PANC-1 injection treated with the control, RO, CQ or RO + CQ at day 28. D. Body weight (g) of the nude mice with sciatic nerve BxPC-3 injection treated with the control, RO, CQ or RO + CQ. No significant body weight difference was detected at day 1,7,14,21 and 28. E. Sciatic nerve score of the nude mice with sciatic nerve BxPC-3 injection treated with the control, RO, CQ or RO + CQ. RO + CQ could increase the sciatic nerve score at day 28. F. The paw span (mm) of the nude mice with sciatic nerve BxPC-3 injection treated with the control, RO, CQ or RO + CQ at day 28. G. PANC-1 nerve invasion specimens were collected on Day 28. Black arrow: sciatic nerve embedded in the tumor bulk. H. PANC-1 nerve invasion tumor size (mm) (tumor image: Fig. 7G) (* p < 0.05). I. BxPC-3 nerve invasion specimens were collected on Day 28. Black arrow: sciatic nerve embedded in the tumor bulk. J. BxPCc-3 nerve invasion tumor size (mm) (tumor image: Fig. 7I) (* p < 0.05). K. HE staining of the PANC-1 nerve invasion model. Black arrow: sciatic nerve. L. HE staining of the BxPC-3 nerve invasion model. Black arrow: sciatic nerve. M. HE staining of liver metastasis in the PANC-1 sciatic nerve invasion model. Black arrow: tumor metastasis. N. Tumor formation, liver metastasis, lung metastasis and peritoneal nodule metastasis status of the PANC-1 and BxPC-3 nerve invasion models