Fig. 8

HuD is required for in vivo tumor growth. A Schematic representation of neuroblastoma xenograft tumor implantation and doxy induction. B Xenograft tumor images (control or silenced HuD). C and D Tumor volume measure for IMR-32 and SK-N-SH neuroblastoma tumor (control or silenced HuD). E and F Representative tumor weight data for IMR-32 and SK-N-SH xenograft. G and H Protein expression analysis in the tumor (control or silenced HuD). HuD, GFP (for HuD shRNA induction), Ki67 (for proliferation), and GL2 (neuroblastoma marker) expression was detected; the scale bar corresponds to 50 μm. Relative quantifications are shown (right). I and J Validation of HuD, GRB-10, ARL6IP1, NeuroD1 and miR375 signals in neuroblastoma IMR-32 and SK-N-SH xenograft (control or silenced HuD). Data are presented as mean ± SEM; t test: *p < 0.05, **p < 0.01, ***p < 0.001