Fig. 3

GLS1 depletion sensitizes HNSCC cells to CPI-613. A The effect of GLS1 knockdown on PDH and α-KGDH protein levels determined by Western blotting. B The effect of GLS1 knockdown on PDH activity in HNSCC cells. C The effect of combined GLS1 knockdown and CPI-613 treatment on PDH activity in HNSCC cells. D The effect of combined GLS1 knockdown and CPI-613 treatment on HNSCC cell viability. E The effect of combined GLS1 knockdown and CPI-613 treatment on HNSCC cell viability in SeedEZ™ 3D scaffold on Day 14 after treatment. Representative fluorescence images and quantitative data from three independent experiments are shown in the left and right panels, respectively. *p < 0.05, **p < 0.01