Fig. 4

Suppression of miR-23a-3p potentiated sorafenib response both in vivo and in vitro. A Mice with orthotopic implantation of Scramble and 23a-KO cells (n = 6) and the signal intensity in HCC in 4 weeks. B Images and the weight of HCC-bearing livers. Yellow circles indicate HCC tumours. C Cleaved caspase-3 staining in the orthotopic HCC section. Cells with green staining are apoptotic cells. D The IC₅₀ value of sorafenib after miR-23a-3p and Anti-miR-23a transfection in MHCC97L and PLC for 24 h. Mean ± SD of IC₅₀ was displayed and P-value of comparison between groups was shown. E and F The effect of miR-23a-3p in cell apoptosis determined by FACS analysis using Annexin V-FITC/7-AAD double staining kit and immunoblotting