Fig. 7

A competitive relationship between NESs mediated INPP5F-CRM1 interaction and INPP5F-importin-α interaction. A Schema of the NESs mutant and the three truncations (Trunc1, Trunc2 and Trunc3) of INPP5F. The prediction of NLSs was obtained from http://nls-mapper.iab.keio.ac.jp/cgi-bin/NLS_Mapper_form.cgi, and the prediction of NESs was obtained from http://www.cbs.dtu.dk/services/NetNES/ . B-D Cells were transfected with wild type (WT), NESs mutant or the three truncations. (B) Sub-cellular localization of different INPP5F was detected by immunofluorescence. C The nuclear localization of different INPP5F was validated by western blot. D The expression of INPP5F-related downstream molecules was detected by western blot. E Immunofluorescent staining displayed CRM1 and INPP5F mainly co-localized in the cell nucleus. F Huh7 Cells were transfected with Flag-tagged INPP5F WT and NESs mutant. Cell lysates were immunoprecipitated with anti-Flag antibody and immunoblotted with anti-CRM1 antibody. G Huh7 Cells were transfected with Flag-tagged INPP5F WT, NLSs Trunc2 and NESs mutant. Cell lysates were immunoprecipitated with anti-Flag antibody and immunoblotted with anti-Importin-α as well as anti-CRM1 antibody