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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: The RNA-binding protein GRSF1 promotes hepatocarcinogenesis via competitively binding to YY1 mRNA with miR-30e-5p

Fig. 1

GRSF1 is frequently increased in HCC and promotes HCC in vitro. A Analyses of GRSF1 expression in HCC and nontumor tissues in TCGA datasets. B Correlation between GRSF1 expression levels and prognosis for HCC patients in TCGA datasets. C Analyses of GRSF1 mRNA expression levels in 120 HCC and paired noncancerous tissues. D GRSF1 expression levels in HCC cell lines and THLE-2 cells. E GRSF1 protein expression in 10 HCC samples and paired noncancerous tissues. F Kaplan–Meier analysis showing the association between GRSF1 expression and prognosis in 120 HCC patients. G GRSF1 expression levels in MHCC-97H and Hep3B cells were decreased following transfection with sh-GRSF1 lentivirus. H-J MTT (H), colony formation (I), and Transwell (J) assays showed that GRSF1 knockdown suppressed HCC cell proliferation, colony-forming ability and migration. The invaded cells in Transwell assays were quantified by counting the cells in 10 random fields (magnification, 200×). K FCM assays showed that GRSF1 knockdown enhanced MHCC-97H and Hep3B cell apoptosis. Values are the mean± SEM (= 3); *< 0.05, **< 0.01

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