Fig. 2

GRSF1 enhanced YY1 expression by interacting with YY1 mRNA. A YY1 mRNA expression levels in 120 HCC and paired noncancerous tissues. B GRSF1 and YY1 expression levels were decreased in GSRF1-deficient HCC cells. C RIP assays showing a direct interaction between GRSF1 and YY1. UBC mRNA (which does not bind GRSF1) was used as a negative control, LMNB1 (a known RNA target of GRSF1) was used as a positive control, and GAPDH was used as a background control mRNA. D Silencing GRSF1 markedly shortened the half-life of YY1 mRNA in HCC cells. E Separation of the full-length YY1 3`UTR into different overlapping fragments. F Pull-down assays showing that GRSF1 specifically interacts with the 3`UTR-2205 of YY1. G Pull-down assays showing that GRSF1 associates with the 3`UTR 2663-2847 of YY1. H Luciferase reporter assays showing that GRSF1 interacts with the YY1 3`UTR. I Luciferase reporter assays suggesting that GRSF1 interacts with the 3`UTR-2205 of YY. J Luciferase reporter assays showing that GRSF1 interacts with the 3`UTR-2663 of YY1. Values are the mean± SEM (n=3). *p < 0.05, **p < 0.01