Fig. 4

miR-30e-5p inhibited YY1 and hepatocarcinogenesis by binding to the 3'UTR of YY1. A miR-30e-5p and its putative binding sequence in the YY1 3'-UTR. B miR-30e-5p expression was decreased in HCC tissues. C miR-30e-5p expression in MHCC-97H and Hep3B cells was increased upon transfection with pre-miR-30e-5p but was not regulated by YY1 overexpression. D Luciferase reporter gene assay showing that miR-30e-5p overexpression decreased YY1 luciferase activity when combined with the wt YY1 3'-UTR. E YY1 expression was decreased, followed by increased miR-30e-5p expression, and upregulated upon transfection with the ov-YY1 vector in HCC cells. F-I Increased miR-30e-5p expression suppressed the colony formation (F), proliferation (G, H) and migration (I) ability of HCC cells, and the antitumor function of miR-30e-5p was counteracted by YY1 overexpression. Values are the mean± SEM (n = 3). *p < 0.05, **p < 0.01