Fig. 6

KU-177 decreases MM cell proliferation and PI resistance induced by AHSA1/HSP90 in vitro. A-B Effects of 48 h treatment with KU-177 on cell viability of ARP1 (A) and H929 (B) WT and AHSA1-OE cells. C-E Effects of 48 h treatment with KU-177 (50 μM) on cell apoptosis of ARP1 (C) and H929 (D) WT and AHSA1-OE cells. F Effects of 48 h treatment with KU-177 (30 μM) on cell viability of flow MRD-positive peripheral cells from first diagnosed and relapsed MM patients. H-K Co-IP assay revealed that 48 h treatment with KU-177 (30 μM) inhibited the interaction between HSP90 and AHSA1 in AHSA1-OE cells. L-N Proteasome activity assay showed that 48 h treatment of KU-177 (30 μM) inhibited proteasome activity in AHSA1 WT/OE cells (L), PSMD2 WT/OE cells (M) and ANBL6 WT/DR (N) cells. The data are expressed as mean ± SD.*p<0.05, **p<0.01, ***p<0.001. The data are expressed as mean ± SD.*p<0.05, **p<0.01, ***p<0.001