Fig. 1
From: Adiponectin triggers breast cancer cell death via fatty acid metabolic reprogramming
Effect of globular adiponectin on cellular lipid reservation in breast cancer cells. A-C MCF-7 (A), T47D (B), and MDA-MB-231 (C) cells were treated with gAcrp (1 μg/mL) for indicated time periods. Cells were then incubated with Bodipy 493/503 for 30 min. Neutral lipid accumulation was determined as mean fluorescence intensity (MFI) using flow cytometry analysis. D-E MCF-7 (D) and MDA-MB-231 (E) cells were treated with gAcrp for 24 h, followed by labeling of lipid droplets with Nile red. Representative images for gAcrp-treated and control cells were shown along with quantification of lipid droplet area in respect to DAPI, in which the lipid droplet areas were determined by total area of red fluorescence using Image J software (scale bar: 20 μm). * denotes p < 0.05 compared to control cells (n=3 except where specifically indicated in Figures)