Fig. 7

Knockdown of GAL-1 rescues CSC-like properties induced by ZFP64 overexpression in GC cells. A-B Knockdown of GAL-1 diminished the inhibitory effect of ZFP64 on nab-paclitaxel-induced apoptosis. HGC-27 and MGC-803 cells overexpressing ZFP64 and control cells were transfected with NC or shGAL-1 for 24 h and then treated with nab-paclitaxel for 48 h before the apoptosis rate was examined. C Knockdown of GAL-1 increased the sensitivity of nab-paclitaxel in ZFP64 overexpression cells. D-E The enhanced sphere formation ability induced by ZFP64 overexpression in GC cells was abrogated by GAL-1 silencing. F High CD44 level in ZFP64-overexpression cells was decreased by GAL-1 silencing. G Western blot analysis of the activation of ERK and AKT signaling after the co-transfection of shGAL-1 and ZFP64 overexpression constructs into GC cells. H ERK inhibitor (U0126) 、AKT inhibitor (MK2206) and GAL-1 inhibitor (OTX008) inhibited sphere formation of ZFP64 overexpressing GC cells. Data are presented as the means±SEM. * P < 0.05; ** P < 0.01; *** P < 0.005; **** P < 0.001