Fig. 4

Acsl3 knockout enhances autophagy in KPC mice. A Immunoblot analysis of p62 in pancreatic tumours of KPC;Acsl3^+/+ and KPC;Acsl3^−/− mice. B Representative images of immunofluorescence staining of LC3B (green, left), p62 (green, middle) and nuclei (blue) of pancreatic cancer sections from KPC;Acsl3^+/+ and KPC;Acsl3^−/− mice; Scale bar 10 μm. Representative images of hematoxylin & eosin (H&E) stained tissue depicting healthy (top), PanINs (middle) and PDAC (bottom) are shown on the right; Scale bar: 50 μm. PanINs: Pancreatic intraepithelial neoplasias. C Quantifications of cytoplasmic punctae of LC3B (top) and p62 (bottom) from (B), n = 5 mice/group. Graphical data are shown as the mean ± s.d. Statistical analyses were done using two-way ANOVA. **** P < 0.0001