Fig. 6
From: Restriction of extracellular lipids renders pancreatic cancer dependent on autophagy
ACSL3 knockdown and autophagy blockade cooperate in suppressing tumor formation in vivo. A Tumor burden of PANC-1 xenografts transduced with a shRNA against ACSL3 (shACSL3 #1), before subcutaneous injection into the right flank of the mice and treated with HCQ (60 mg/kg) as indicated by the arrow. Tumors were measured with a caliper every 2 days; n = 5 mice/group. B Kaplan-Meier graph showing survival of the mice from (A). Death was considered when the tumors reached the endpoint of 1000 mm3; n = 5 mice/group. Graphical data are shown as the mean ± s.e.m. Statistical analyses were done using one-way ANOVA or log-rank (Mantel–Cox) test (where indicated)