Fig. 5

A High-level overview of altered pathways in miR-423-5p mimic compared to control samples. For each pathway, scores were generated using a linear combination (a weighted average) of gene expression values that represent pathways using nSolver Advance analysis module V 4.0. The pathways are listed on the horizontal axis and samples are listed vertically. The green colour indicates low scores; the red colour indicates high scores. Scores are displayed on the same scale via Z-transformation. Clustering of the scores were performed using unsupervised hierarchical clustering (Euclidean distance, complete linkage) and visualized using a Morpheus (Broad Institute, MA, USA). B Histogram graph of differentially expressed (DE) genes (down−/up-regulated) involved in Pan Cancer Progression pathways that were analysed by NanoString Technologies. Data are shown as Log2 fold compared to control (cells transduced with the empty vector). C Volcano plot showing differential expression of key genes upon miRNA KD in comparison to the control group. Y–axis represent corrected pValue (Benjamini–Yekutieli). The X-axis represents log2 fold change. The vertical dotted line indicates absolute fold change of 2 and the horizontal line denotes a corrected p- value of 0.05. Significant genes are labelled and colour-coded as red. D Histogram graph of differentially expressed (DE) genes validated by qPCR in PC3 prostate cancer cells. *P = 0.0263 (VEGF B), ***p = 0.0002 (CXCL8), **p = 0.0011 (AGR2), *p = 0.0143 (LOX). E Histogram graph of expression of genes involved in EMT