Table 1 Primers used for recombinant pmirGLO Dual Luciferase Plasmids production
From: MiR-423-5p prevents MALAT1-mediated proliferation and metastasis in prostate cancer
MALAT1 Binding site A Forward | MALAT1 Binding site A Reverse |
CTAGAGAAGCCTCAGCTCGCCTGAAGGCAGGTCCCCTCTGACGCCTCCGGGAGCCCAGT | CTAGACTGGGCTCCCGGAGGCGTCAGAGGGGACCTGCCTTCAGGCGAGCTGAGGCTTCT |
MALAT1 Binding site B Forward | MALAT1 Binding site B Reverse |
CTAGACCTTTTTTTAAGATTTTTCAGGTACCCCTCACTAAAGGCACCGAAT | CTAGATTCGGTGCCTTTAGTGAGGGGTACCTGAAAAATCTTAAAAAAAGGT |
MALAT1 Mutated Binding site Forward | MALAT1 Mutated Binding site Reverse |
CTAGAGAAGCCTCAGCTCGCCTGAAGGCATTTTTTTTGACGCCTCCGGGA | CTAGATCCCGGAGGCGTCAAAAAAAAATGCCTTCAGGCGAGCTGAGGCTTC |