Fig. 1
The clinical significance of macrophages infiltration and aPKCι in human CCA. A. Reclassification of Myeloid Immune Cell in Cholangiocarcinoma based on Single Cell RNA Sequencing. B. The expression of TAMs markers and some cancer-promoting genes in each Macrophages subtypes. C. IHC was used to examine the levels of aPKCɩ (1:200) and macrophage markers such as CD68 (1:150), CD80 (1:500), and CD206 (1:400) in 70 paired CCA and paratumor tissues. Representative images are shown. Scale bar, 20 μm. D. Quantification of aPKCɩ and macrophage markers expression level in paired CCA and paratumor tissues. E. Kaplan-Meier analysis indicating the correlation between the prognosis of CCA patients and the expression of aPKCɩ, CD68, CD80 and CD206