Fig. 4
IP-MS indicates that PCMT1 interacts with LAMB3. A The heatmap of identified interacting proteins in our four IP-MS samples groups. B KEGG pathway enrichment of interacting with the PCMT1 protein set. C The proteins identified by IP-MS were subjected to KEGG-based clustering analysis. D IP-western blot analysis validated the interaction of PCMT1 and LAMB3. E The number of living cells after spheroid formation was calculated by cell count assay in control or LAMB3-silenced PCMT1-OE SKOV3 cells. F LAMB3 was knocked down in control or PCMT1-OE SKOV3 cells, and cell migration was determined and quantified. (scale bar: 200 μm; Data are shown as mean ± SEM of 3 independent experiments. Data were statistically analyzed with Student's t-test. *P < 0.05; **P < 0.01; ***P < 0.001)