Fig. 5

Cell invasion and adhesion are enhanced by supernatant from PCMT1-OE cells and inhibited by a PCMT1 blocking antibody. A The culture supernatant of PCMT1-OE SKOV3 cells was collected and immunoprecipitated using an anti-HA antibody, followed by western blot analysis. B The culture supernatant of sgControl and sgPCMT1 SKOV3 cells were collected and subjected for analysis of PCMT1 expression by western blot. C-D The media of PCMT1 knockout cells were replaced with the supernatants (SN) from PCMT1-KO (sgPCMT1) SKOV3 cells, control (sgControl) SKOV3 cells and PCMT1-OE cells. The cells were then continuously cultured for 24 h and examined for cell invasion (C) and cell adhesion (D). E and F The supernatant of PCMT1-OE SKOV3 cells was collected and incubated with PCMT1 antibody for 4 h and then used for PCMT1-KO SKOV3 cell culture. After 24 h, cell invasion (E) and adhesion (F) were determined (scale bar: 200 μm; Data are shown as mean ± SEM of 3 independent experiments. Data were statistically analyzed with Student's t-test. *P < 0.05; **P < 0.01; ***P < 0.001)