Fig. 3

PYCR1 inhibition by pargyline and siPYCR1 reduces MM cell viability in vitro. OPM-2 and RPMI-8226 were cultured in a hypoxic environment during 48 h (pargyline) and 72 h (siRNA). SiRNA-mediated knockdown was established using 20 nM siPYCR1 and/or 50 nM siPYCR2. A Viability was measured by CellTiter Glo assay after cells were treated with increasing doses of pargyline (n = 3). B Pargyline-induced apoptosis was measured using flow cytometry by staining for Annexin V and 7-AAD (n = 3). C Viability was measured by CellTiter Glo assay after treatment with siRNA for PYCR1 and/or PYCR2 (n = 5). D Apoptosis was measured using flow cytometry by staining for Annexin V and 7-AAD after treatment with siRNA for PYCR1 and/or PYCR2 (n = 5). All experiments were performed in hypoxic conditions. Significance was determined by Mann–Whitney U test and one-way ANOVA. * p ≤ 0.05, ** p ≤ 0.01